Description:
PNGaseAisarecombinantamidase,whichcleavesbetweentheinnermostGlcNAcandasparagineresiduesofhighmannose,hybrid,andshortcomplexoligosaccharidessuchasthosefoundinplantandinsectcellsfromN-linkedglycoproteinsandglycopeptides.PNGaseAdiffersfromPNGaseFinthatitcleavesN-linkedglycanswithorwithoutα(1,3)-linkedcorefucoseresidues.
SubstrateSpecificity PNGaseAhydrolyzesN-glycanchainsfromglycoproteins/peptidesregardlessofthepresenceofxyloseorfucose.[x=HorManorGlcNAc].
PNGaseAhydrolyzesN-glycanchainsfromglycoproteins/peptidesregardlessofthepresenceofxyloseorfucose.[x=HorManorGlcNAc].ProductSource
ClonedfromOryzasativa(rice)andexpressedinPichiapastoris(1).ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
|  | Storeat(°C) | Concentration | 
| GlycoBuffer3 | -20 | 10X | 
| GlycoproteinDenaturingBuffer | -20 | 10X | 
| NP-40 | -20 | 10% | 
Notes:
PNGaseAisactiveonbothglycoproteinsandglycopeptides.PNGaseAcannotcleavelargerN-glycanssuchasthosefromFetuin,Fibrinogen,IgG,LactoferrinandTransferrin.PNGaseAisabletocleavehighmannoseN-glycanstructuresfromMan3uptoMan9.Reactionsmaybescaled-uplinearlytoaccommodatelargerreactionvolumes.