Description:

?
Peptide-N-GlycosidaseF,alsoknownasPNGaseF,isanamidasethatcleavesbetweentheinnermostGlcNAcandasparagineresiduesofhighmannose,hybrid,andcomplexoligosaccharidesfromN-linkedglycoproteins(1)
DetailedSpecificity: PNGaseFisnotabletocleaveN-linkedglycansfromglycoproteinswhentheinnermostGlcNAcresidueislinkedtoanα1-3Fucoseresidue.Thismodificationismostcommonlyfoundinplantandsomeinsectglycoproteins.
PNGaseFisnotabletocleaveN-linkedglycansfromglycoproteinswhentheinnermostGlcNAcresidueislinkedtoanα1-3Fucoseresidue.Thismodificationismostcommonlyfoundinplantandsomeinsectglycoproteins.ProductSource
PNGaseFispurifiedfromFlavobacteriummeningosepticum(3)anditisfreeofproteasesandEndoFactivities.ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
|  | Storeat(°C) | Concentration | 
| GlycoproteinDenaturingBuffer | -20 | 10X | 
| GlycoBuffer2 | -20 | 10X | 
| NP-40 | -20 | 10% | 
Notes:
SincePNGaseFactivityisinhibitedbySDS,itisessentialtohaveNP-40presentinthereactionmixture.Whythisnon-ionicdetergentcounteractstheSDSinhibitionisunknownatpresent.Todeglycosylateanativeglycoprotein,longerincubationtimeaswellasmoreenzymemayberequired.PNGaseFwillnotcleave?N-linkedglycanscontainingcoreα1-3Fucose.Typicalreactionconditions:PleaseseeProtocols