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Millipore/MAB3757 | Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9/MAB3757/100 µL
  • Millipore/MAB3757 | Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9/MAB3757/100 µL

Millipore/MAB3757 | Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9/MAB3757/100 µL

價(jià)格: ￥3576.00 市場(chǎng)價(jià): 5960.00
貨號(hào): MAB3757
品牌: Millipore
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    • Description
      CatalogueNumberMAB3757
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-VEGFReceptor-3Antibody,extracellulardomain,clone9D9F9
      AlternateNames
      • VEGFR3
      • VEGFR-3
      • ReceptortyrosineKinaseVEGFR-3
      BackgroundInformationVascularendothelialgrowthfactorreceptor-3(VEGFR-3)anditsligands,vascularendothelialgrowthfactor-C(VEGF-C)and-D(VEGF-D),arethemajormoleculesinvolvedindevelopmentalandpathologicallymphangiogenesis.{Bando,2004}.JussilaetalreportedthatVEGFR3antibodiesspecificallystainendothelialcellsoflymphaticvesselsandvesselsaroundtumonrssuchaslymphomaandinsitubreastcarcinoma.{Jussila,1998}.Interestingly,thespindlecellsofseveralcutaneousnodularAIDS-associatedKaposi"ssarcomasandtheendotheliumaroundthenoduleswerealsoVEGFR-3positive.ThefirstspecificmolecularMarkerforthelymphaticendotheliumshouldprovideausefultoolfortheanalysisoflymphaticvesselsinmalignanttumorsandtheirmetastasesandthecellularoriginanddifferentiationofKaposi"ssarcomas.{Jussila,1998}.
      ProductInformation
      FormatAscites
      Control
      • Humanlymphatictissues
      • VEGFR-3isnotexpressedinHEVsofthelymphnodes.{Jussila,1998].Inlymphomasstainingisseenmostlyincollapsedvesselsinthecortexoflymphnodesinfiltratedbythelymphomacells.Theantibodyspecificallydetectslymphaticendotheliuminhumanlymphnodeandotherorganssuchastonsil.SeeJussilaetal,1998forcompletetissuecharacterization.
      PresentationUnPurifiedascitesfluidcontaining0.1%sodiumazideasapreservative.
      StorageandShippingInformation
      StorageConditionsMaintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationAnti-VEGFReceptor-3Antibody,extracellulardomain,clone9D9F9isanantibodyagainstVEGFReceptor-3foruseinFC,IF,IH(P),IP&WB.
      KeyApplications
      • FlowCytometry
      • Immunofluorescence
      • Immunohistochemistry(Paraffin)
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesImmunohistochemistry:frozencryosections,5μmcryosectionswereair-driedandfixedincoldacetonefor10minutes.Blockedwith5%normalhorseserumandthenincubatedwith9D9for2hoursatroomtemperatureinahumidchamber.Typicaldilutionswillrangefrom1:100-1:1000dependinguponlottiter.Detectioniswithenhancedmethods(ABCorpolyHRP)andenzymaticsubstratesforbestresults.

      Immunohistochemistryinparaffin:5μmthicklightlyfixedformalintissueswereprocessedthroughdecreasingethanolconcentrations,washedwithwater,thenheatedinamicrowavein10mMsodiumcitratepH6.0at780Wfor5minutes,followedby450Wfor10minutes.Thesectionswerethenincubatedinmethanolcontaining30%hydrogenperoxidefor30minutesandprocessedfurtherlikethecryosections.{Jussila,1998}.

      WesternBlot:antibodyrecognizesVEGFR-3onreducedsamplesofHUVECandHDMECcells{primayhumanumbilicalveinendothelialcells&primaryhumandermalmicrovascularendothelialcells}.Thebandsseenatmolecularweight195kDaand125kDarepresenttheuncleavedandproteolyticallycleavedformsofVEGFR3.Thebandat175kDaisconsideredtobeintracellular,unglycosylatedprecursor{Bando,2004}.

      Immunoprecipitation:1-5μLper300-500μLofcelllysate.UseproteinAorrabbitanti-mouseIgGcaptureforbestresults.

      ELISA:9D9F9hasbeenusedasthedetectionantibodyforaVEGFR-3ELISA{Bando,2004}.

      FlowCytometry:reactiveontransfectedNIH-3T3cellsexpressinghVEGFR3,Othercelllinesuntested.{Jussila,1998}.

      Optimaldilutionswillneedtobedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenRecombinantlyproduced,purified,hVEGFR-3extracellulardomainproteinfrombaculovirusculture{Jussila,1998}.
      Epitopeextracellulardomain
      Clone9D9F9
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificitySpecificforhumanVEGFR-3.WesternblotandELISAdemonstratethattheantibodyishighlyspecificforVEGFR-3withnocross-reactivitywithhumanVEGFR-1orVEGFR-2protein{Bando,2004}.CrossreactivitywithotherspeciesVEGFR-3proteinsuntested.
      IsotypeIgG1
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • VEGFR3
      PurificationMethodUnpurified
      UniProtNumber
      MolecularWeight175kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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