Description:
OneTaq?HotStartQuick-Load?2XMasterMixwithGCBufferisanoptimized,ready-to-useblendofTaqandDeepVentR?DNAPolymerasescombinedwithanaptamer-basedinhibitor.ThisenzymeblendisideallysuitedtoPCRapplicationsfromGC-richtemplates,includingpureDNAsolutions,bacterialcolonies,andCDNAproducts.The3′→5′exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).Thehotstartnatureoftheenzymeoffersconveniencewithdecreasedinterferencefromprimerdimersandsecondaryproducts.TheconvenientmastermixformulationcontainsdNTPs,MgSO4,buffercomponentsandstABIlizersaswellastwocommonlyusedtrackingdyesforDNAgels.Ona1%agarosegelin1XTBE,XyleneCyanolFFmigratesat~4kbandTartrazinemigratesat~10bp.Bothdyesarepresentinconcentrationsthatdonotmaskco-migratingDNAbands.
 AmplificationofaselectionofsequenceswithvaryingGCcontentfromhumangenomicDNAusingOneTaqHotStartDNAPolymerase.Thepresenceorabsenseofanextendedroomtemperatureincubationdoesnotaffectperformance.AmpiconsizeisindicatednexttogelandGCcontentisindicatedbelowgel.MarkerMisthe1kbDNALadder.
AmplificationofaselectionofsequenceswithvaryingGCcontentfromhumangenomicDNAusingOneTaqHotStartDNAPolymerase.Thepresenceorabsenseofanextendedroomtemperatureincubationdoesnotaffectperformance.AmpiconsizeisindicatednexttogelandGCcontentisindicatedbelowgel.MarkerMisthe1kbDNALadder. ??
??Highlights
ProductSource
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andanE.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
|  | Storeat(°C) | Concentration | 
| OneTaq?HighGCEnhancer |  | 10X | 
Notes:
ProductspecificationsforindividualcomponentsintheOneTaqHotStartQuick-Load2XMasterMixwithGCBufferareavailableseparately.OneTaqHotStartQuick-Load2XMasterMixwithGCBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°C.OneTaqHotStartQuick-Load2XMasterMixwithGCBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.