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Sigma-Aldrich/HiTrap® rProtein A Fast Flow/GE17-5080-02/
  • Sigma-Aldrich/HiTrap® rProtein A Fast Flow/GE17-5080-02/

Sigma-Aldrich/HiTrap® rProtein A Fast Flow/GE17-5080-02/

價(jià)格: ¥25989.15 市場(chǎng)價(jià): 33785.90

貨號(hào): GE17-5080-02
品牌: Sigma-Aldrich
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    • Properties:

      Related Categories Analytical/Chromatography, GE Healthcare Life Sciences Protein Sample Preparation Formats, HiTrap & PD-10 Cartridges, HiTrap System/syringe, Molecular Biology,
      More...
      shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
      packaging   pack of 5 × 5 mL
      mfr. no.   GE Healthcare, 17-5080-02
      parameter   <20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
        42 psi
      bed size   16 mm × 25 mm
      bed volume   5 mL
      column I.D.   16 mm
      matrix   4% cross-linked agarose
      particle size   60-165 μm
      average diameter   90 μm (d50v)
      cleaning   2 - 11 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
      working range   3 - 10
      capacity   ~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
      suitability   suitable for bioprocess medium
      storage temp.   2-8°C

      Description:

      Analysis Note

      To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

      Features and Benefits

      • Quick, convenient preparative purification of monoclonal antibodies.
      • Well-established protein A-based medium used in many approved MAb processes.
      • Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
      • Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.

      General description

      HiTrap® rProtein A FF columns are prepacked with rProtein A Sepharose® Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

      The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose® Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

      Legal Information

      HiTrap is a registered trademark of GE Healthcare

      Sepharose is a registered trademark of GE Healthcare

      Safety:

      Symbol 
      GHS02  GHS02
      Signal word 
      Warning
      Hazard statements 
      H226
      Precautionary statements 
      P210-P370 + P378
      RIDADR 
      UN1170 - class 3 - PG 3 - Ethanol, solution
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