| StorageConditions | Storeat2°to8°C,forupto6months. 
 ChemiconHRPconjugatedantibodiesmaybedilutedupto1:16,000inEPAIIBuffer.Dilutedconjugatesarestableforatleast3monthswhenstoredat2°to8°C.ThebufferissuitablefordirectuseinELISA.
 
 EPAIIBufferiscompatIBLewithblockingagentssuchasskimmilkpowder,Tween20andnormalserum.HoweverHRPconjugatesshouldnotbestoredinthepresenceoftheseblockingagents.Theyshouldbeaddedjustpriortouseintheassay.
 
 PRECAUTIONS:
 
 HRPconjugatesshouldbedilutedandstoredundercleanconditionstoreducethelevelofmicrobialchallenge.
 
 SodiumazidemustNOTbeaddedtoEPAIIBuffer.
 
 AvoidcontaminationofHRPconjugatesolutionswithheavymetalions,aromaticchlorocarbonsorhypochlorousacids.Avoidcontainersmadeofsodaglassorpolystyrene.Storageofdilutedconjugatesinborosilicateglassisrecommended.
 
 EfficientwashingstepsshouldfollowHRPconjugateincubations.InELISA,atleastfourthoroughwashcyclesarerecommended.
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