Description:
ThePh.D.-12PhageDisplayPeptideLibraryisbasedonacombinatoriallibraryofrandomdodecapeptidesfusedtoaminorcoatprotein(pIII)ofM13phage(1–6).Thedisplayedpeptide(12-mer)isexpressedattheN-terminusofpIII,i.e.,thefirstresidueofthematureproteinisthefirstrandomizedposition.Thepeptideisfollowedbyashortspacer(Gly-Gly-Gly-Ser)andthenthewild-typepIIIsequence.Thelibraryconsistsofapproximately109electroporatedsequencesamplifiedoncetoyieldapproximately100copiesofeachsequencein10μlofthesuppliedphage.
CitationsforPhageDisplay

PanningwithapentavalentpeptidelibrarydisplayedonpIII.KitComponents
Thefollowingreagentsaresuppliedwiththisproduct:
|  | Storeat(°C) | Concentration | 
| Streptavidin,lyophilized | -20 |  | 
| Biotin | -20 | 10mM | 
| E.coliK12ER2738 | -70 |  | 
| -28glllSequencingPrimer(22-mer) | -20 | 1pmol/μl | 
| -96glllSequencingPrimer(20-mer) | -20 | 1pmol/μl | 
| Ph.D.™-12PhageDisplayPeptideLibrary | -20 | 1x1013pfu/ml | 
| Streptavidin,lyophilized | -20 |  | 
| Biotin | -20 | 10mM |