Synonym:
TSKgel® Ether-5PW HPLC Column
Properties:
| Related Categories |
Analytical/Chromatography,
HPLC,
HPLC & FPLC Columns for Biopolymer Separations,
Hydrophobic Interaction (HIC) Columns,
TSKgel Ether-5PW Column (1000 ? pore size),
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mfr. no.
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Tosoh Ether-5PW, 08643
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parameter
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≤4 M salt concentration
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≤50% organic solvent
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10-50 °C temperature
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L × I.D.
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2.5 cm × 6 mm
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matrix
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polymer particle platform
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matrix active group
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ether phase
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particle size
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20 μm
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operating pH
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2 - 12
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mode of chromatography
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Hydrophobic interaction (HIC)
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Description:
General description
Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000 ?‚??pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2??M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
TSKgel Guardgel kits contain:
• 5 mL Guardgel packing material
• Column holder, consisting of external reducing union, cap, frit, 2 ferrules
• Column, 25 mm x 9.5 mm OD x 6 mm ID, 2 μm frit at 10 mm depth, 1/16"" exit
• 2 Replacement frits
• Capillary tubing, 5 cm x 1/16"" OD x 0.01"" ID
• Nuts and ferrules, 10-32 threads for 1/16"" tubing, 2 each
• All hardware is 316 stainless steel
Dimensions for Guardgel kits for 21.5 mm ID columns:
• Column, 35 mm x 12.75 mm OD x 10 mm ID, 10 μm frit at 20mm depth, 1/16"
• Capillary tubing, 5 cm x 1/16"" OD x 0.02"" ID
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Legal Information
TSKgel is a registered trademark of Tosoh Corporation
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