Analytical/Chromatography,
BIOshell A400 Protein C4, 3.4 μm HPLC Columns,
BIOshell HPLC Columns,
C4 for Biopolymers,
C4, 3.4 μm Capillary Columns,
Capillary Dimensions,
HPLC,
HPLC & FPLC Columns for Biopolymer Separations
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material
stainless steel hardware
description
Shell thickness (0.2 μm)
Solid Core (3.0 μm)
feature
endcapped
extent of labeling
0.4% carbon loading
parameter
600 bar max. pressure
90 °C max. temp.
application(s)
HPLC: suitable
L × I.D.
15 cm × 100 μm
surface coverage
4.2 μmol/m2
matrix
spherical silica particle platform
matrix active group
C4 (butyl) phase
particle size
3.4 μm
pore size
400 ?
surface area
15 m2/g
operating pH range
2 - 9
suitability
suitable for L26 per USP
mode of chromatography
Reversed phase
Description:
General description
BIOshell™ 400 ? Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 ? pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 ? fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.
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