Properties:
| Related Categories |
Analytical/Chromatography,
Ascentis Express Columns for Fast HPLC,
Ascentis Express HILIC, 5μm HPLC Columns,
HILIC, 5 μm Columns,
HPLC,
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|
feature
|
endcapped: no
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parameter
|
60 °C temp. range
|
|
application(s)
|
HPLC: suitable
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|
L × I.D.
|
5 cm × 4.6 mm
|
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impurities
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<5 ppm metals
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matrix
|
Fused-Core particle platform
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matrix active group
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silica phase
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particle size
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5 μm
|
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pore size
|
90 ? pore size
|
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operating pH
|
2-9
|
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suitability
|
suitable for L3 per USP
|
|
mode of chromatography
|
Hydrophilic interaction (HILIC)
|
Description:
Frequently Asked Questions
Frequently Asked Questions
are available for this Product.
General description
Ascentis Express 5 μm HILIC is a high-speed, high-performance liquid chromatography column based on a 5-micron Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shorter diffusion paths in the 0.6-micron thick porous shell around and an overall particle size of ~5-microns. The bare silica stationary phase of Ascentis Express 5 μm HILIC provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversed-phase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds.
Column Characteristics
The Fused-Core particle has a surface area of ~ 100 m2/g and an average pore size of 90?. The Fused-Core particles are 30% to 50% heavier than commercially available totally porous particles due to the density of the solid cores. Therefore, the effective surface area per column is similar to columns packed with totally porous particles having surface areas in the 160-200 m2/g range.
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Legal Information
Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
Safety:
RIDADR
NONH for all modes of transport