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Millipore/17-613 | ChIPAb+ p53 - ChIP Validated Antibody and Primer Set/17-613/25 assays25 assays per kit. ~1 μg per chr
  • Millipore/17-613 | ChIPAb+ p53 - ChIP Validated Antibody and Primer Set/17-613/25 assays25 assays per kit. ~1 μg per chr

Millipore/17-613 | ChIPAb+ p53 - ChIP Validated Antibody and Primer Set/17-613/25 assays25 assays per kit. ~1 μg per chr

價格: ￥4800.00 市場價: 8000.00
貨號: 17-613
品牌: Millipore
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    • Description
      CatalogueNumber17-613
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+p53-ChIPValidatedAntibodyandPrimerSet
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+p53setincludesthep53antibodyraisedagainstrecombinanthumanp53,thenegativecontrolantibody(mousenormalIgG),andqPCRprimersflankinganSp1bindingsite(towhichp53canberecruited1)withinthehumanp21(WAF1/CIP1/CDKN1A)promoter,amplifyinga105basepairPCRproduct.Thep53andnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofp53associatedchromatin.
      ProductInformation
      FormatPurified
      Control
      • IncludednegativecontrolantibodymousenormalIgGandcontrolprimersspecificforhumanp21(WAF1/CIP1/CDKN1A)promoter.
      PresentationAnti-p53(mousemonoclonalIgG).Onevialcontaining25μgofofproteinApurifiedmouseIgG2ain25μLofstoragebuffer(PBS,pH7.4,containing0.05%sodiumazideand1mg/mLBSA).Storeat-20°C.

      NormalMouseIgG.Onevialcontaining25μgofnormalmouseIgGin25μLvolume.Storeat-20°C.

      ChIPPrimersp21.Onevialcontaining75μLof5μMofeachprimerspecificforaregionofthehumanp21(WAF1/CIP1/CDKN1A)promoter.Storeat-20°C.
      FOR:GTGGCTCTGATTGGCTTTCTG
      REV:CTGAAAACAGGCAGCCCAAG
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt
      Applications
      ApplicationThisChIPAb+p53-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • WesternBlotting
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitationAnalysis:
      SonicatedchromatinpreparedfromuntreatedoractinomycinDtreated(30ng/mL,6hrs.)U2OScells(3X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing1μgofmouseAnti-p53andtheMagnaChIP?G(Cat.#17-611)Kit.Immunoprecipitationofp53associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimersp21flankingthehumanp21promoterthatcontainsanSp1bindingsite(Pleaseseefigures).
      FoldIncreaseisaratioofnormalizedmeanIPquantitiesextractedfromstandardcurvesderivedfrominputsofeachchromatinsample.Aspreviouslypublished,p53associationwiththisregionofthep21promoterincreasesuponactinomycinDtreatment.

      ChromatinImmunoprecipitationAnalysis:
      SonicatedchromatinpreparedfromuntreatedorUVtreated(6hrs,50joules/m2.)U2OScells(3X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing1μgofmouseAnti-p53andtheMagnaChIP?A(Cat.#17-610)Kit.
      Immunoprecipitationofp53associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimersp21flankingthehumanp21promoterthatcontainsanSp1bindingsite(Pleaseseefigures).
      FoldIncreaseisaratioofnormalizedmeanIPquantitiesextractedfromstandardcurvesderivedfrominputsofeachchromatinsample.p53immunoprecipitableactivityassociatedwiththispromoterincreaseswithUVtreatmentasobservedinotherstudies.
      BiologicalInformation
      HostMouse
      SpecificityRecognizesp53inwildtypeandmutantformsofhumanorigin.
      IsotypeIgG
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • P53
      • TP53
      PurificationMethodProteinAPurfied
      UniProtNumber
      MolecularWeight53kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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