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當(dāng)前位置： Millipore >>> Millipore/04-242 | Anti-p53 (CT) Antibody, clone E47, rabbit monoclonal/04-242/100 µL

Millipore/04-242 | Anti-p53 (CT) Antibody, clone E47, rabbit monoclonal/04-242/100 µL

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Description
CatalogueNumber	04-242
Description	Anti-p53(CT)Antibody,cloneE47,rabbitmonoclonal
AlternateNames	AntigenNY-CO-13 Phosphoproteinp53 Tumorsuppressorp53 p53antigen p53transformationsuppressor p53tumorsuppressor transformation-relatedprotein53 tumorproteinp53
BackgroundInformation	Thep53geneismutatedinapproximatelyhalfofallhumancancers.Itisinvolvedinthecellularresponsetocytotoxicstresses,andtogetherwithp19ARF,inducesexpressionofp21Cip1,tocausecellcyclearrest.Inaddition,p53isabletoinduceapoptosis,bothbytranscriptionalandnon-transcriptionalmechanisms.Theamino-terminal83aminoacidsofp53containthetransactivationdomain,aswellastheregioninvolvedintranscription-independentgrowthsuppression.Thecarboxy-terminalregioncontainstheDNA-bindingdomain,whichisregulatedbythreephosphorylationevents,andpotentiallybyacetylationalso.

ProductInformation
Format	Unpurified

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20oCfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

Applications
Application	PleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.Anti-p53(C-terminus)Antibody,cloneE47isanantibodyagainstp53(C-terminus)foruseinIP,IC,FC&WB.
KeyApplications	Immunoprecipitation Immunocytochemistry FlowCytometry WesternBlotting
ApplicationNotes	FlowCytometry: A1:50dilutionofapreviouslotwasusedinFC. Immunoprecipitation: A1:50dilutionofapreviouslotwasusedinIP. Immunocytochemistry: A1:100dilutionofapreviouslotwasusedinIC.

BIOLOGicalInformation
Immunogen	KLHconjugatedlinearpeptidecorrespondingtop53.
Epitope	C-terminus
Clone	E47
Host	Rabbit
Specificity	Thisantibodyrecognizesp53attheC-terminus.
Isotype	IgG
SpeciesReactivity	Human
SpeciesReactivityNote	Proventoreactwithhuman.Notexpectedtoreactwithmouseorrat.
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_000546.4
EntrezGeneSummary	Thisgeneencodestumorproteinp53,whichrespondstodiversecellularstressestoregulatetargetgenesthatinducecellcyclearrest,apoptosis,senescence,DNArepair,orchangesinmetabolism.p53proteinisexpressedatlowlevelinnormalcells andatahighlevelinavarietyoftransformedcelllines,whereit"sbelievedtocontributetotransformationandmalignancy.p53isaDNA-bindingproteincontainingtranscriptionactivation,DNA-binding,andoligomerizationdomains.Itispostulatedtobindtoap53-bindingsiteandactivateexpressionofdownstreamgenesthatinhibitgrowthand/orinvasion,andthusfunctionasatumorsuppressor.Mutantsofp53thatfrequentlyoccurinanumberofdifferenthumancancersfailtobindtheconsensusDNAbindingsite,andhencecausethelossoftumorsuppressoractivity.Alterationsofthisgeneoccurnotonlyassomaticmutationsinhumanmalignancies,butalsoasgermlinemutationsinsomecancer-pronefamilieswithLi-Fraumenisyndrome.Multiplep53variantsduetoalternativepromotersandmultiplealternativesplicinghavebeenfound.Thesevariantsencodedistinctisoforms,whichcanregulatep53transcriptionalactivity.[providedbyRefSeq].
GeneSymbol	p53 FLJ92943 LFS1 P53 TRP53
Non-ReactiveSpecies	Rat Mouse
PurificationMethod	Unpurified
UniProtNumber	P04637
UniProtSummary	FUNCTION:Actsasatumorsuppressorinmanytumortypes;inducesgrowtharrestorapoptosisdependingonthephysiologicalcircumstancesandcelltype.Involvedincellcycleregulationasatrans-activatorthatactstonegativelyregulatecelldivisionbycontrollingasetofgenesrequiredforthisprocess.Oneoftheactivatedgenesisaninhibitorofcyclin-dependentkinases.ApoptosisinductionseemstobemediatedeitherbystimulationofBAXandFASantigenexpression,orbyrepressionofBcl-2expression.Ref.48 CofactorBinds1zincionpersubunit. SUBUNITSTRUCTURE:InteractswithAXIN1.ProbablypartofacomplexconsistingofTP53,HIPK2andAXIN1Bysimilarity.BindsDNAasahomotetramer.InteractswithhistoneacetyltransferasesEP300andmethyltransferasesHRMT1L2andCARM1,andrecruitsthemtopromoters.Invitro,theinteractionofTP53withcancer-associated/HPV(E6)viralproteinsleadstoubiquitinationanddegradationofTP53givingapossIBLemodelforcellgrowthregulation.Thiscomplexformationrequiresanadditionalfactor,E6-AP,whichstablyassociateswithTP53inthepresenceofE6.C-terminusinteractswithTAF1,whenTAF1ispartoftheTFIIDcomplex.InteractswithING4andthisinteractionmaybeindirect.FoundinacomplexwithCABLES1andTP73.InteractswithHIPK1,HIPK2,andP53DINP1.InteractswithWWOX.MayinteractswithHCVcoreprotein.InteractswithUSP7andSYVN1.InteractswithHSP90AB1.InteractswithCHD8,leADIngtorecruithistoneH1andpreventtransactivationactivityBysimilarity.InteractswithARMC10,BANP,CDKN2AIPandE4F1. SUBCELLULARLOCATION:Cytoplasm.Nucleus.Endoplasmicreticulum.Note=InteractionwithBANPpromotesnuclearlocalization.Ref.51Ref.56 DOMAIN:Thenuclearexportsignalactsasatranscriptionalrepressiondomain.Ref.34 PTM:Acetylated.AcetylationofLys-382byCREBBPenhancestranscriptionalactivity.DeacetylationofLys-382bySIRT1impairsitsabilitytoinduceproapoptoticprogramandmodulatecellsenescence. PhosphorylationonSerresiduesmediatestranscriptionalactivation.PhosphorylatedbyHIPK1Bysimilarity.PhosphorylationatSer-9byHIPK4increasesrepressionactivityonBIRC5promoter.PhosphorylatedonThr-18byVRK1,whichmaypreventtheinteractionwithMDM2.PhosphorylatedonThr-55byTAF1,whichpromotesMDM2-mediateddegradation.PhosphorylatedonSer-46byHIPK2uponUVirradiation.PhosphorylationonSer-46isrequiredforacetylationbyCREBBP.PhosphorylatedonSer-392followingUVbutnotgammairradiation.PhosphorylateduponDNAdamage,probablybyATMorATR.PhosphorylatedonSer-15uponultravioletirradiation;whichisenhancedbyinteractionwithBANP. DephosphorylatedbyPP2A.SV40smallTantigeninhibitsthedephosphorylationbytheACformofPP2A.Ref.51Ref.24Ref.26Ref.31Ref.35Ref.36Ref.40Ref.41Ref.49Ref.53Ref.58Ref.59Ref.60 MaybeO-glycosylatedintheC-terminalbasicregion.StudiedinEB-1cellline.Ref.28 UbiquitinatedbySYVN1,whichleadstoproteasomaldegradation.Ref.56 MonomethylatedatLys-372bySETD7,leadingtostabilizeitandincreasetranscriptionalactivation.MonomethylatedatLys-370bySMYD2,leadingtodecreaseDNA-bindingactivityandsubsequenttranscriptionalregulationactivity.Lys-372monomethylationpreventstheinteractionwithSMYD2andsubsequenctmonomethylationatLys-370.Ref.50Ref.54Ref.55 DISEASE:TP53isfoundinincreasedamountsinawidevarietyoftransformedcells.TP53isfrequentlymutatedorinactivatedinabout60%ofcancers. DefectsinTP53areinvolvedinesophagealsquamouscellcarcinoma(ESCC)[MIM:133239].ESCCisatumoroftheesophagus. DefectsinTP53areacauseofLi-Fraumenisyndrome(LFS)[MIM:151623].LFSisanautosomaldominantfamilialcancersyndromethatinitsclassicformisdefinedbytheexistenceofaprobandaffectedbyasarcomabefore45yearswithafirstdegreerelativeaffectedbyanytumorbefore45yearsandanotherfirstdegreerelativewithanytumorbefore45yearsorasarcomaatanyage.OtherclinicaldefinitionsforLFShavebeenproposed(Ref.95andRef.98)andcalledLi-Fraumenilikesyndrome(LFL).Inthesefamiliesaffectedrelativesdevelopadiversesetofmalignanciesatunusuallyearlyages.Fourtypesofcancersaccountfor80%oftumorsoccurringinTP53germlinemutationcarriers:breastcancers,softtissueandbonesarcomas,braintumors(astrocytomas)andadrenocorticalcarcinomas.Lessfrequenttumorsincludechoroidplexuscarcinomaorpapillomabeforetheageof15,rhaBDomyosarcomabeforetheageof5,leukemia,Wilmstumor,malignantphyllodestumor,colorectalandgastriccancers.Ref.74Ref.75Ref.76Ref.77Ref.78Ref.97Ref.99Ref.104Ref.105 DefectsinTP53maybeassociatedwithnasopharyngealcarcinoma[MIM:161550];alsoknownasnasopharyngealcancer. DefectsinTP53arefoundinBarrettmetaplasia;alsoknownasBarrettesophagus.Itisaconditioninwhichthenormallystratifiedsquamousepitheliumoftheloweresophagusisreplacedbyametaplasticcolumnarepithelium.Theconditiondevelopsasacomplicationinapproximately10%ofpatientswithchronicgastroesophagealrefluxdiseaseandpredisposestothedevelopmentofesophagealadenocarcinoma. DefectsinTP53areinvolvedinheadandnecksquamouscellcarcinomas(HNSCC)[MIM:275355]. DefectsinTP53areinvolvedinoralsquamouscellcarcinoma(OSCC).Cigarettesmokeisaprimemutagenicagentincanceroftheaerodigestivetract. DefectsinTP53areacauseoflungcancer[MIM:211980]. DefectsinTP53areacauseofchoroidplexuspapilloma[MIM:260500].Choroidplexuspapillomaisaslow-growingbenigntumorofthechoroidplexusthatofteninvadestheleptomeninges.Inchildrenitisusuallyinalateralventriclebutinadultsitismoreofteninthefourthventricle.Hydrocephalusiscommon,eitherfromobstructionorfromtumorsecretionofcerebrospinalfluid.Ifitundergoesmalignanttransformationitiscalledachoroidplexuscarcinoma.Primarychoroidplexustumorsarerareandusuallyoccurinearlychildhood.Ref.108 DefectsinTP53areacauseofoneformofhereditaryadrenocorticalcarcinoma(ADCC)[MIM:202300].ADCCisararechildhoodtumor,representingabout0.4%ofchildhoodtumors,withahighincidenceofassociatedtumors.ADCCoccurswithincreasedfrequencyinpatientswiththeBeckwith-Wiedemannsyndrome[MIM:130650]andisacomponenttumorinLi-Fraumenisyndrome[MIM:151623].Ref.107 SIMILARITY:Belongstothep53family.
MolecularWeight	53kDa

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